Centrin Scaffold in Chlamydomonas reinhardtii Revealed by Immunoelectron Microscopy
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چکیده
منابع مشابه
Centrin scaffold in Chlamydomonas reinhardtii revealed by immunoelectron microscopy.
In the flagellate green alga Chlamydomonas reinhardtii the Ca(2+)-binding EF-hand protein centrin is encoded by a single-copy gene. Previous studies have localized the protein to four distinct structures in the flagellar apparatus: the nucleus-basal body connector, the distal connecting fiber, the flagellar transitional region, and the axoneme. To explain the disjunctive distribution of centrin...
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Chlamydomonas cells excise their flagella in response to a variety of experimental conditions (e.g., extremes of temperature or pH, alcohol or detergent treatment, and mechanical shear). Here, we show that flagellar excision is an active process whereby microtubules are severed at select sites within the transition zone. The transition zone is located between the flagellar axoneme and the basal...
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Organelles are intracellular compartments which are themselves compartmentalized. Biogenic and metabolic processes are localized to specialized domains or microcompartments to enhance their efficiency and suppress deleterious side reactions. An example of intra-organellar compartmentalization is the pyrenoid in the chloroplasts of algae and hornworts. This microcompartment enhances the photosyn...
متن کاملCentrin plays an essential role in microtubule severing during flagellar excision in Chlamydomonas reinhardtii
Previously, we reported that flagellar excision in Chlamydomonas reinhardtii is mediated by an active process whereby microtubules are severed at select sites within the flagellar-basal body transition zone (Sanders, M. A., and J. L. Salisbury. 1989. J. Cell Biol. 108:1751-1760). At the time of flagellar excision, stellate fibers of the transition zone contract and displace the microtubule doub...
متن کاملThe NIT1 promoter allows inducible and reversible silencing of centrin in Chlamydomonas reinhardtii.
An inverted repeat corresponding to parts of the centrin gene of Chlamydomonas reinhardtii was placed downstream of the NIT1 promoter, which is induced by ammonium starvation. After induction, transformants developed centrin deficiency as assayed by immunofluorescence, Western blotting, and Northern blotting. The effect was reversible, demonstrating that the NIT1 promoter allowed controlled RNA...
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ژورنال
عنوان ژورنال: Eukaryotic Cell
سال: 2005
ISSN: 1535-9778,1535-9786
DOI: 10.1128/ec.4.7.1253-1263.2005